In vitro multiplication of the semi-arid forest tree, Balanites aegyptiaca (L.) Del.

Authors

  • Mansor Ndoye1, Ismaïla Diallo2 and Yaye Kène Gassama/Dia1 1 Laboratoire de biotechnologies, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop Dakar, B.P. 5005, Dakar, Sénégal. 2 ISRA-DRPF, Route des Pères Maristes, B.P. 2312, Dakar, Sénégal. Author

Keywords:

Balanites aegyptiaca, micropropagation, rooting.

Abstract

Procedures were developed for micropropagation of Balanites aegyptiaca using axillary bud explants
obtained from mature trees. Cultures were established in Murashige and Skoog (MS) medium
supplemented with 2.5 mg/l 6-benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA). The
effects of kinetin on shoot growth and proliferation in vitro was also investigated. Results show that
shoot multiplication required 2.5 mg/l of BAP. Shoot length was significantly affected by the presence
of BAP or 6-furfurylaminopurine (Kin). Rooting of shoots in vitro was achieved on MS medium
containing 20 mg/l of the auxin, indolebutyric acid (IBA). Rooted shoots acclimated and were
successfully transferred into soil, with 48% of the plantlets surviving.

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Published

2022-01-28

Issue

Vol. 9 No. 1 (2022): 2022

Section

Articles

How to Cite

In vitro multiplication of the semi-arid forest tree, Balanites aegyptiaca (L.) Del. (2022). African Journal of Geography and Regional Planning, 9(1), 1-4. https://ijpp.org/journal/index.php/AJGRP/article/view/151