Investigation of drug susceptibility in S. aureus isolated from burn patients in Missan city

Abstract

Twenty one isolates of Staphylococcus aureus collected from Alsader a hospital – Missan then were analyzed
between (October and December in 2013). The samples taken in order to determine the bacterial profile and
antibiotic susceptibility. Isolates of S. aureus were tested against 8 differrent antibiotics and crude of
methanol extracts of Capsicum annuum L.var. (Solanaceae) by a disk diffusion method. 100% of the isolates
were resistant to the Penicillin, 95% resistant to Lincomycin , 90.4% Tetracyclin. 90.4% Rifampin, 80.9%
resistant to Nitrofuraantion and 57.1% resistant to Bacteriacin were found to be the least effective antibiotics
while 9.5% resistant to Amicacine and 38.09% resistant to Novobiocine where more effective antibiotics
against (MRSA) S. aureus. The minimum inhibitory concentration (MIC) (mg/ml) of the alcoholic were also
determined. Also the antimicrobial suscepitlity of isolates where tested against methanol extract of C.
annuum with different concentration (500, 250, 125, 62.5 μġ/ml) detected by agar well diffusion methods. The
alcoholic extracts proved to be more effective than aqueoous extracts antimicrobials against (MRSA) S.
aureus. Finally cytotoxicity evaluating towards human RBC, the results revealed these crud extracts of C.
annuum have not any cytotoxicity in all coocentrations. The good antimicrobial potency of the methanol
extract of C. annuum plants indicates the tretment of (MRSA) as an alternative to the costly antibiotics.