Appraisal of the 15 N-isotope dilution and 15 N natural abundance methods for quantifying nitrogen fixation by flood-tolerant green manure legumes

Authors

  • Eklou A. Somado1* and Ronald F. Kuehne2 1 Africa Rice Center (WARDA), 01 Bp 2031 Cotonou, Benin Republic. 2 University of Goettingen, Department of Crop Science, Tropical Agronomy Division, Grisebachdtr. 6, D-37077 Goettingen Germany. Author

Keywords:

Aeschynomene afraspera, Biological nitrogen fixation, 15 N-isotope dilution, 15 N natural abundance.

Abstract

Quantification of biological nitrogen fixation (BNF) is fundamental to identifying potential green
manure legumes for their enhanced nitrogen contribution to sustained crop production. A screenhouse
experiment compared BNF by Aeschynomene afraspera L. as affected by phosphorus, using the
15Nisotope dilution and the 15 N methods. Rice ( Oryza sativa L.) was grown as a non -fixing plant.
Phosphorus significantly increased the percent N derived from the atmosphere (% Ndfa), whereby the
15 N method tended to overestimate BNF contribution on average by 20% compared to the 15 N dilution.
Data confirmed rice as a non-fixing plant for estimation of BNF by flood-tolerant legumes.

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Published

2021-04-29

Issue

Vol. 9 No. 1 (2021): 2021

Section

Articles

How to Cite

Appraisal of the 15 N-isotope dilution and 15 N natural abundance methods for quantifying nitrogen fixation by flood-tolerant green manure legumes. (2021). International Journal of Manures and Fertilizers, 9(1), 1-5. https://ijpp.org/journal/index.php/ijmf/article/view/91