Association between steroid hormone receptors and PSA gene expression in breast cancer cell lines
Keywords:
PSA, steroid hormone receptor, prostate cancer, breast cancer.Abstract
The prostate specific antigen (PSA) gene is a member of the human kallikrein gene family and is known that
to be tightly regulated by androgens in the male prostate The presence of PSA is strongly associated with
presence of steroid hormone receptors. The aim of this research was to show differential expression and
association between steroid hormone receptors and PSA gene expression in breast cancer cell lines. The cell
lines investigated were steroid receptor-negative breast carcinoma cell lines BT- 20 and HBT-100 and the
steroid hormone receptor-positive breast carcinoma cell lines BT-474, ZR-75-1, MDA-MB-453 and MFM-233.
Others include BG-1 (ovarian), MFE-296 (endometrium), HBT-161 (ovarian), HBT-173 (ovarian), A-427 (lung),
SK-MES-1 (lung), NCI-H460 (lung), MIA PaCa (pancreas), and Colo320 (Colon) cell lines. All cell lines were
cultured as confluent and then harvested. Stimulation test was carried out using steroid compound. The
concentration of estrogen receptor (ER) and progesterone receptor (PR) in all tumor cell lines were measured
with the abbot enzyme immunoassay kit. Among cell lines tested, only steroid receptor positive cells were
able to produce PSA at the protein and the mRNA level in response to stimulatory effects of steroid
hormones. Our data suggest that the expression of the PSA gene in different cancer cell lines may depends
on the presence of steroid hormone receptors.
